Recent Publications
Peptide-based biocoatings for corrosion protection of stainless steel biomaterial in a chloride solution.
Authors
Muruve, Cheng YF, Feng Y, Liu T, Muruve DA, Hassett DJ, Irvin RT
Details
Abstract
In this work, PEGylated D-amino acid K122-4 peptide (D-K122-4-PEG), derived from the type IV pilin of Pseudomonas aeruginosa, coated on 304 stainless steel was investigated for its corrosion resistant properties in a sodium chloride solution by various electrochemical measurements, surface characterization and molecular dynamics simulation. As a comparison, stainless steel electrodes coated with non-PEGylated D-amino acid retroinverso peptide (RI-K122-4) and D-amino acid K122-4 peptide (D-K122-4) were used as control variables during electrochemical tests. It was found that the D-K122-4-PEG coating is able to protect the stainless steel from corrosion in the solution. The RI-K122-4 coating shows corrosion resistant property and should be investigated further, ...
A Putative ABC Transporter Permease is Necessary For Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa Under Aerobic, Anaerobic, Planktonic or Biofilm Conditions
Authors
Cameron McDaniel, Warunya Panmanee, Shengchang Su, Renuka Kapoor, Kevin Cox, Andrew Paul, Gee Lau, Seung-Hyun Ko, Joel Mortensen, Joseph S. Lam, Daniel Muruve and Daniel Hassett
Details
Pseudomonas aeruginosa (PA) is an important airway pathogen of cystic fibrosis and chronic obstructive disease patients. Multiply drug resistant PA is becoming increasing prevalent and new strategies are needed to combat such insidious organisms. We have previously shown that a mucoid, mucA22 mutant PA is exquisitely sensitive to acidified nitrite (A-NO2-, pH 6.5) at concentrations that are well tolerated in humans. Here, we used a transposon mutagenesis approach to identify PA mutants that are hypersensitive to A-NO2-. Among greater than 10,000 mutants screened, we focused on PA4455, in which the transposon was found to disrupt ...
Peptide-Mediated PEGylation of Polysulfone Reduces Protein Adsorption and Leukocyte Activation
Authors
Noah G.G. Muruve, Y. Frank Cheng, Yuanchao Feng, Tao Liu, Daniel A. Muruve, Daniel J. Hassett, Randall T .Irvin
Details
Abstract
The exposure of blood to bio-incompatible materials used for dialysis triggers leukocyte activation and protein adsorption. We describe a single-step, post-manufacturing method for surface modification to create biomaterials used in medical devices and dialysis with altered surface characteristics. Peptides derived from the receptor-binding domain of the type IV pilin of Pseudomonas aeruginosa were synthesized using L and D-amino acids to generate L-K122-4, enantiomer D-K122-4, and D-retroinverso RI-K122-4 peptides. L-K122-4, D-K122-4, and RI-K122-4 peptides, but not control peptides, bound durably to the surfaces of materials used in medical devices and dialysis including silicone and polysulfone. D-K122-4 ...
